血清源酸热稳定多肽对伤口组织细胞增殖作用的研究

The Study of Acid/heat Stable Polypeptides on the Proliferation of Wound Tissue Cells

目的：研究一种对酸热稳定的多肽（Ａｃｉｄ／ｈｅａｔ Ｓｔａｂｌｅ Ｐｏｌｙｐｅｐｔｉｄｅｓ，ＡＨＳＰ）制剂对伤口组织细胞增殖反应的作用及机理。方法：利用从猪血清中分离出的一组对酸热稳定的多肽（ＡＨＳＰ）以及从国外购得的牛血清除大分子蛋白后的多肽成分（Ｓｏｌｃｏｓｅｒｙｌ），观察两种多肽制剂在培养的伤口组织修复细胞及人表皮细胞增殖和细胞总蛋白合成能力中的作用。结果：当Ｓｏｌｃｏｓｅｒｙｌ和ＡＨＳＰ的浓度低于７％时，其对伤口修复细胞和人离体培养的表皮细胞的增殖和蛋白合成能力随浓度增加而增加，浓度为５％时，两者对细胞活力的促进作用明显强于相应浓度的小牛血清对照组；而与 １０％的小牛血清对照组相比无明显差异。 Ｓｏｌｃｏｓｅｒｙｌ和 ＡＦＳＰ的浓度为 ５％～ ７％时，曲线变化较为平缓，当浓度上升为 １０— １２％时， Ｓｏｌｃｏｓｅｒｙｌ和 ＡＨＳＰ组与相应浓度的小牛血清对照组相比有明显的抑制作用。结论：在伤口愈合过程中ＡＰＳＰ可能是通过提高细胞的酶活性，促进细胞的蛋白合成，进而刺激伤口组织细胞增生并促进伤口愈合的。

objective: In the experiment, the effects and its possible mechanisms of two polypeptides with different concentrations on the proliferation wound tissue cells were studied. Methods: One polypeptides stable to both acid and heat was isolated from pig serum, nominated as acid and/or heat stable polypeptides (AHSP). Another polypeptides was purchased from abroad(called Solcoseryl), which was iaolated from cow serum, We then observed the effects of the both polypeptides on the proliferation and the synthesis of total protein of cultured wound repair cells and human epider mal cells. Results: It was found that both Solcoseryl and AHSP could effectively promote the growth of cultured human epithelium and wound heal ing cells when their concentrations were lower than 7 %, At 5 %, both polypeptides could improve the cell growth and were much more effective than those control groups using the corresponding concentrations of fetal calf serum.While it is not statistically different with the conrtol group of 10% FCS. When the concentrations of Solcoseryl and AHSP were 5 -- 7 %, their effects Were almost the same. When their concentrations reached to 10 12%,both presented remarkable inhibitory effects than those control groups of fetal calf serum of the corresponding concentrations. Conclusion: AHSP may play the roles of various growth factors during the process of wound healing, Its mechanisms of enhancing wound healing might be through the proliferation of wound healing cells.