杞黄酮类化合物分离及抗氧化研究

Extraction Total Flavonolid from Lycium Barbarum L.(TEL) and its Scaveging Effect on Hydroxyl and Super Oxygen Radicals *

对枸杞叶中提取的黄酮类化合物用 Ｈ Ｐ Ｌ Ｃ 法进行分析鉴定。发现９ 个组分。在产生氧自由基的 Ｆｅｎｔｏｎ 体系和 Ｘａｎ／ Ｘ Ｏ 体系中，枸杞总黄酮在终浓度为７５ ～２００ｍｇ／ Ｌ时，对· Ｏ Ｈ 的清除率为２０ ％ ～７２ ％ ；在 Ｘａｎ／ Ｘ Ｏ 体系中，浓度为０ ～２１７ｍｇ／ Ｌ 时， Ｏ－２· 清除率为０ ％ ～５１ ％ 。均存在剂量效应关系。

The TFL was extracted from the leave of Lycium Barbarum L.and contained 9 components displayed by HPLC.The scavenging effects of TFL were studied by using ESR spin trapping technique in radicals generated systems of Fenton reaction and xanthine/xanthine oxidase (Xan/XO).The result showed that TFL(0-217mg/L)could scavenge hydroxyl and super oxygen radicals and the svavenging rates were 20 72% and 0-51% respectively.