菟丝子含药血清促进骨髓间充质干细胞增殖的效应及机制

Effect and mechanism of dodder medicated serum on MSCs proliferation in vitro

目的:探讨菟丝子含药血清体外对骨髓间充质干细胞(MSCs)增殖的影响及机制研究。方法:分离、培养大鼠MSCs,菟丝子含药血清诱导培养MSCs 72h后,MTT法检测大鼠MSCs增殖情况;RT-PCR检测增殖过程中MSCs不同细胞因子mRNA的表达;Real-time PCR检测骨形态蛋白-2(BMP-2)mRNA表达;Western-blot检测BMP-2蛋白表达。结果:10%菟丝子含药血清具有显著促进MSCs增殖的作用(P<0.05),并显著促进BMP-2 mRNA的表达(P<0.05)。结论:10%菟丝子含药血清促进MSCs增殖的作用,可能与其促进BMP-2 mRNA表达有关。

Objective：To investigate the effect and mechanism of dodder medicated serum on bone marrow mesenchymal stem cells（MSCs） proliferation in vitro.Methods：The dodder medicated serum-induced proliferation effect on MSCs was mesured by MTT;different cytokine mRNA expression was detected by RT-PCR,BMP-2 mRNA expression was analyzed by Real-time PCR;BMP-2 protein expression was detected by western blot.Results：Compared with the blank serum group,10%dodder medicated serum can significantly promote the proliferation of MSCs,and markedlly improved BMP-2 mRNA expression.Conclusion：10% dodder medicated serum can promote the proliferation of MSCs,and the mechanism involves the stimulation of BMP-2 mRNA expression.