参环毛蚓不同部位细胞原代培养及鉴定

Study on Primary Culture and Identification of Different Organizations Cells from Pheretima Aspergillum

目的：筛选出参环毛蚓体内细胞培养的最佳部位,为建立参环毛蚓细胞原代培养方法提供实验基础。方法：分别提取参环毛蚓的皮、肠道、嗉囊、前列腺细胞进行培养,观察其细胞生长。结果：参环毛蚓的表皮和肠道细胞经培养后,细胞均有生长,并发现肠道细胞生长良好,5~6天后基本达到融合,细胞呈多边鹅卵石样,透明度及折光性强。另发现参环毛蚓的嗉囊、前列腺等部位的细胞没有增长迹象。结合形态学鉴定,原代培养及传代的细胞98%以上为肠上皮细胞。结论：参环毛蚓的肠道是建立细胞系的首选材料,采用此方法分离培养的肠上皮细胞数量多,均一性生长佳,可重复性强。

Objetive：To explore the best method which the cell culture derived from the organizations by pheretima aspergillum.It provide the experimental basis for study of primary cell cultural method of the pheretima aspergillum.Methods：The cell culture derived from the epidermis,the enteral,the crop and the prostates of pheretima aspergillum.The cells were culture by the diferent tissue and organ.in order to observe the cell growth.Results：The cell growth was quickly with the the enteral,after 4-5days,the cultured cells reached almost complete confluence,displaying the typical cobblestone layout,gooddiaphaneity and strong refraction.In addition,the cellhave no growth with the crop and the prostates,the cell growth was slowly with the epidermis refraction.According to the results of cell morphology,the purity of primary culture and secondary cultured enterocytes eached 98%.Conclusion：This culture system can yield rich andhomogeneous cells,offering a satisfactory experiments mode.