呼吸道感染肺气虚证大鼠血气分析IL-8及肺组织病理学改变

Changes of Blood Gas Analysis IL-8 and Pulmonary Pathology in Rats with Lung Qi Deficiency Syndrome Due to Respiratory Tract Infection

目的:研究呼吸道感染肺气虚证大鼠的症候表现和血气指标变化、肺匀浆IL-8及肺组织病理学改变的相关性。方法:用呼吸道生物被膜菌(铜绿假单胞菌)以滴鼻法,辅以寒冷疲劳刺激引起大鼠呼吸道慢性感染,诱导肺气虚证的发生,观察证候表现、检测血气指标、IL-8及肺组织病理学改变。并设一组补肺益气治疗组予以反证。结果:模型组大鼠支气管及周围肺组织慢性炎症明显。pH、PO2、SaO2%下降,PCO2、肺匀浆IL-8升高,与正常对照组比较差异有显著性(P<0.01),补肺益气治疗组支气管炎症不明显,血气指标、肺匀浆IL-8接近正常组(P>0.05)。结论:呼吸道感染肺气虚证大鼠症候表现和支气管及周围肺组织慢性炎症以及pH、PO2、SaO2%下降,PCO2升高相关,肺匀浆IL-8升高和肺部炎症的发生相关。

Objective：To investigate the correlation between the symptoms and blood gas indicators,IL-8 level in lunghomogenates and pulmonary pathology in rats with Lung Qi deficiency syndrome caused by respiratory tract infection.Method：To develop rats with Lung Qi deficiency syndrome model with intranasal inoculation of respiratory biofilm bacterial（pseudomonas aeruginosa）,combined with cold and fatigue,and to observe the symptoms and detcet blood gas indicators,IL-8 level in tissues and pulmonary pathology,and the group of BufeiYiqi treatment was set up to further prove it.Result：Chronic inflammation in bronchitis and surrounding pulmonary tissue was obvious in model group.pH,PO2 and SaO2% were significantly lower in model group than those in control group while PCO2 and IL-8 in lunghomogenates were significantlyhigher than those in control group（P0.01）.In Bufei Yiqi treatment group,chronic bronchitis was not obvious and blood gas indicators and IL-8 of lunghomogenates were close to those of the control group（P0.05）.Conclusion：Rats with Lung Qi deficiency syndrome due to respiratory tract inflections was related to chronic inflammation in bronchitis and surrounding pulmonary tissues,the decrease of pH,PO2,SaO2% and the increase in PCO2.The increase in IL-8 of Lunghomogenate solution was related to Lung inflammation.