耐多药相关蛋白基因表达与肺癌病理特征关系的研究

The expression of MRP gene relates to the pathological features of lung cancer

目的 探讨耐多药相关蛋白（ Ｍ Ｒ Ｐ） 基因表达与肺癌病理特征的关系。方法 应用逆转录聚合酶链反应（ Ｒ Ｔ Ｒ Ｃ Ｒ） 技术对３５ 例原发性肺癌、癌旁组织及２８ 枚淋巴结中 Ｍ Ｒ Ｐ 基因的表达进行检测、分析。结果 肺癌组织及癌旁组织中 Ｍ Ｒ Ｐ阳性率分别为５４ ％ 和１４ ％ ，两者比较差异有显著性（ Ｐ＜ ０００５） ；转移淋巴结组织 Ｍ Ｒ Ｐ阳性率为５３ ％ ；各病理类型中不同分化程度肿瘤的 Ｍ Ｒ Ｐ阳性分布不同，术前化疗病例的 Ｍ Ｒ Ｐ阳性率（７８ ％ ） 高于未化疗者（４６ ％ ） 。随诊发现， Ｍ Ｒ Ｐ 阳性者肿瘤复发、转移的发生率（５５ ％ ） 高于 Ｍ Ｒ Ｐ阴性者（１５ ％ ） 。结论 原发性肺癌 Ｍ Ｒ Ｐ 基因表达增高，术前化疗可能起诱导作用。癌组织与其转移淋巴结中 Ｍ Ｒ Ｐ表达具有一致性， Ｍ Ｒ Ｐ的过度表达与肿瘤的大小、临床分型、 Ｔ Ｎ Ｍ 分期无明显关系，但可能提示肿瘤的预后不良。

Objective To evaluate the expression of MRP gene and its relation to pathological features in lung carcinoma Methods A series of 35 resected primary lung cancer tissues from 9 post chemotherapy and 26 untreated patients and adjacent normal lung tissues and 28 lymph node tissues were analyzed for MRP gene expression by RT PCR method Results Positive expression rate of MRP gene was 54% in 35 cases with primary lung cancer, 14% in tissues adjacent to the carcinoma site and the difference was significant ( P <0 005),the positive rate of MRP gene expression in metastatic lymph node tissues was 53% In 9 cases of pre operative chemotherapy, the positive expression rate of MRP gene was higher than that in 26 cases without chemotherapy Nineteen of 35 tumors were found to show positive expression of MRP gene,comprising 11 of 18 adenocarcinoma, 9 of 15 squamous cell cancer The relapse or metastasis rate with MRP positive cases was higher(55%) than that with MRP negative (15%) by follow up of these patients Conclusions There are overexpression of MRP gene in primary lung cancer tissues Preoperative chemotherapy may play inductive role in lung cancer multidrug resistance The positive expression of MRP gene in lung cancer tissues is in accord with its lymph node tissues Positive expression of MRP gene does not necessarily be associated with tumor size,clinical type and TNM stages, but it could be regarded as one poor prognostic factor