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卡氏肺孢子虫肺炎肺灌洗液细胞和生化成分变化的研究 被引量:5

Study on inflammatory response and alteration of biochemical components in BALF from pneumocystis carinii pneumonia
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摘要 目的 研究卡氏肺孢子虫肺炎( P C P) 支气管肺泡灌洗液( B A L F) 中细胞和生化成分的变化及与细菌性肺炎的差别。方法 采用清洁级 Sprague Dawley 大鼠50 只,每周2 次皮下注射泼尼松建立 P C P模型(14 只) ,阴性对照组(6 只) ,细菌性肺炎组(11 只) 为实验组,并设正常对照组(6 只) 。分析 B A L F中细胞及生化成分,并测定其总蛋白( T P) 、白蛋白( A L B) 含量及碱性磷酸酶( A L P) 、乳酸脱氢酶( L D H) 、Ⅳ型胶原酶( M M P2 、 M M P9) 活性。结果  P C P大鼠 B A L F 中细胞总数为(69 ±26) ×106/ L,与阴性对照组(29 ±13) ×106/ L 比较,差异有显著性( P< 001) ,两者多形核细胞( P M Ns) 比例分别为(186 ±60) % 及(17 ±16) % ,差异有显著性( P< 001) 。 P C P 细胞总数与 P M Ns 比例均低于细菌性肺炎组( P< 001) 。 P C P组和阴性对照组 B A L F 中 A L B 分别为(623 ±484)g/ L、(36 ±16)g/ L,两组比较差异有显著性( P< 005) 。 A ? Objective To study the cellular and biochemical changes in airways in pneumocystis carinii pneumonia (PCP) Methods The cellular and biochemical changes in bronchoalveolar lavage fluid (BALF) between PCP and bacterial pneumonia in rat models induced by cortisone acetate(GC) injections were compared The activities of alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and type Ⅳ collagenase(matrix metalloproteinases, MMP 2, MMP 9) in BALF were measured The rats were divided into four groups: (1) normal controls: 6 healthy SD rats; (2) negative control: 6 rats with GC injection over 8 weeks without lung infection; (3) bacterial pneumonia: 11 rats with GC injection over 8 weeks complicating bacterial pneumonia without other pathogens isolated; (4) PCP: 14 rats with GC injection during 8~12 weeks complicating PCP without other pathogens isolated Results In PCP the total cell counts (6 9±2 6)×10 6/L and the percentage of PMNs(18 6±6 0)% in BALF were increased compared with negative control( P <0 01), but were lower than those in bacterial pneumonia group( P <0 01) ALB in BALF in PCP (623±484)g/L was increased compared with negative control (36±16)g/L( P <0 05) and bacterial pneumonia(91±43)g/L( P <0 05) The activities of ALP and LDH in PCP [(217±81) U/L, ( 208±82)U/L respectively] were higher than those in negative control [(75±37)U/L,( 86±61)U/L, P <0 05)] and bacterial pneumonia [(90±45)U/L, (130±75)U/L, P <0 05] The activities of Type Ⅳ collagenase, including MMP 2, MMP 9, were increased in PCP compared with negative control ( P <0.001) and bacterial pneumonia ( P <0.001, P <0.01), and the activities of MMP 2 and MMP 9 had positive correlations with total cell counts in BALF ( r =0 652, P <0 05; r =0 803, P <0 001) Conclusions It suggests that there are differences in the cellular and biochemical aspects of lung inflammation and injury between PCP and bacterial pneumonia
出处 《中华结核和呼吸杂志》 CAS CSCD 北大核心 1999年第9期548-551,共4页 Chinese Journal of Tuberculosis and Respiratory Diseases
基金 上海市教委青年基金 上海市卫生系统百人计划基金
关键词 卡氏肺孢子虫 肺炎 支气管 肺泡灌洗液 肺损伤 Pneumocystis carinii pneumonia Bronchoalveolar lavage fluid Lung injury
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