润燥灵对干燥综合征模型小鼠颌下腺水通道蛋白1、5的影响

The influence of Runzaoling on AQP1 and AQP5 in submaxillary gland of mice with Sjogren syndrome

目的探讨润燥灵汤对干燥综合征模型小鼠颌下腺AQPl及AQP5的影响。方法采用诱导法建立干燥综合征（sjogrensyndrome，SS）模型，按照随机数字表法随机分为6组，空白组（A）、ss模型组（B）、润燥灵低剂量组（C）、润燥灵中剂量组（D）、润燥灵高剂量组（E）、强的松组（F）。A组正常饲养，B组生理盐水灌胃0．5mUd，C、D、E组分别给予17g/kg·d-1、347g/kg·d-1、687g/kg·d-1润燥灵灌胃，F组以强的松0．32mg／d灌胃。应用免疫组化法检测模型小鼠颌下腺组织AQPl和AQP5的表达。结果ss模型组小鼠颌下腺组织中AQPl表达，B组、c组的颌下腺仅见散在浅黄色颗粒：A、D、E、F组小鼠的颌下腺均无明显染色，与B组比较未见明显变化。AOP5的表达，B组小鼠的颌下腺腺泡顶膜、侧膜、基膜和分泌管、导管上皮内均有较强染色的棕黄色颗粒。C组小鼠颌下腺AQP5亦见棕黄色颗粒，但染色较浅；D、E、F组小鼠颌下腺腺泡、分泌管、导管等处染色减弱或消失。结论润燥灵可上调SS模型小鼠颌下腺组织AOP5的表达，增加其饮水量。

Objective To study the influence of the Runzaoling on AQP1 and AQP5 in submaxillary gland of mice with Sjogren syndrome . Methods Sjogren syndrome mice models were setup by innducement method and divided into six groups randomly, including A group （blank group）, B group （model group）, C group （low dose group）, D group （moderate dose group）, E group （high dosage group） and F group （prednisone group） Immunohistochemical method was used to detect the of expressions of AQP1 and AQP5 of submaxillary gland in model mice. Result AQP1 expression showed： scattered flavescent particles were only found in submaxillary gland of group B and group C; AQP5 expression showed： brown-stained granules can be found in gland alveolous teleblem, lateral membrane, and basal lamina of submaxillary gland, secretory duct, and duct epithelia in B group mice; light brawn-stained granules can be found in group C mice; attenuated or disappear stained granules were found in acinus, secretory tube, and duct in D, E, and F group mice. Conclusion Runzaoling can raise the expression of AQP5 in submaxillary gland of SS mice and increase the absorption quantity of water.