蜜环菌对锌的耐性和富集特性

Zinc Tolerance and Accumulation Characteristics of Armillara mellea

研究了锌对蜜环菌Armillaria mellea生长的影响,蜜环菌对于锌的耐性和富集特性,以及锌胁迫下蜜环菌的抗氧化酶的变化情况。结果表明,锌浓度45mg/L下对于蜜环菌菌体的生长有显著促进作用(P<0.05),锌浓度90mg/L以上时,蜜环菌菌体的生长受到抑制(P<0.05)。蜜环菌的子实体萌发和子实体生物量在锌的浓度为45mg/L以下时与对照组无显著性差异(P>0.05),锌的浓度超过90mg/L后子实体不能萌发。培养基锌含量在270mg/L以下时,皮壳状菌丝中锌的含量随培养基中锌浓度的增大而增大。培养基锌含量在135mg/L以下时,菌索中锌的含量随培养基中锌浓度的增大而增大。随着培养基中锌浓度的提高,菌丝和菌索POD、CAT、SOD活性都有增加,PPO的活性则是先升高后降低,而且菌丝与菌索之间抗氧化酶活力有显著差异(P<0.05)。

The growth,zinc tolerance,enrichment features and antioxidant enzyme activity of Armillara mellea under zinc stress were investigated.Our results show that zinc at 45mg/L increased mycelia growth（P0.05）,when higher than 90mg/L,zinc functions to inhibit mycelia growth（P0.05）.Fruiting body formation and fruiting body biomass were not influenced by zinc when zinc content in media were less than 45mg/L（P0.05）,but fruiting body formation was inhibited when zinc content in media were more than 90mg/L.Under the concentration of 270mg/L of supplemented zinc,the content of zinc in crustose aerial hypha of A.mellea elevated along with increasing amount of zinc in media,and under the concentration of 135mg/L of supplemented zinc,the content of zinc in rhizomorphs of A.mellea elevated along with increasing amount of zinc in media.The activity of antioxidative enzymes including peroxidase（POD）,catalase（CAT） and superoxide dismutase（SOD） in crustose aerial hypha and rhizomorphs of A.mellea elevated and the activity of polyphenoloxidase（PPO） increased earlier and depressed later along with increasing amount of zinc in media,but there were observable differences between crustose aerial hypha and rhizomorphs（P0.05）.