转化生长因子β1与白细胞介素6mRNA在糖尿病溃疡创面 …

TRANSFORMING GROWTH FACTOR β 1 AND INTERLEUKIN 6 mRNA EXPRESSION IN WOUND TISSUES OF PATIENTS WITH DIABETIC ULCERS

目的 研究转化生长因子β１（ＴＧＦ－β１）与白细胞介素６（ＩＬ－６）ｍＲＮＡ在糖尿病溃疡创面组织中的变化规律以及与组织修复结局的关系。方法 采用逆转录ＰＣＲ（ＲＴ－ＰＣＲ）技术和病理学方法，研究ＴＧＦ－β１和ＩＬ－６两种生长因子ｍＲＮＡ在糖尿病患者正常皮肤与溃疡创面的不同表达。结果 在模板量相同以及反应条件一致的情况下，溃疡创面组织ＴＧＦ－β１ 和ＩＬ－６ｍＲＮＡ表达的不同。

Objective To study the relationship between the changes of mRNA expression in wound tissues of diabetic ulcers and tissue repair.Methods The mRNA expression of TGF β 1 and IL 6 in eight bioptic samples of diabetic ulcers were detected by RT PCR and pathologic methods, and the surrounding normal skins from the same patients were measured as control group. Results The mRNA expression levels of TGF β 1 were markedly decreased in the diabetic ulcers compared with control group, while the mRNA expression levels of IL 6 were increased at the same reaction conditions.Conclusion The different changes of mRNA expression level of TGF β 1 and IL 6 in wound tissue result in low production and decreased activity of TGF β 1 and IL 6 ,which lower the reparative ability of wound tissue.