碱性成纤维细胞生长因子对脊髓损伤早期保护作用的实验研究

THE EARLY PROTECTIVE EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON ACUTE SPINAL CORD INJURY IN RATS

目的 探讨碱性成纤维细胞生长因子（ｂ Ｆ Ｇ Ｆ）对脊髓损伤的早期保护作用。方法 选３４ 只 Ｓ Ｄ 大鼠，随机分为正常组、对照组和治疗组，采用 Ａｌｌｅｎ 氏技术，以１０ ｇ×２．５ ｃｍ 致伤力造成大鼠 Ｔ８ 急性脊髓损伤，并于损伤平面以下蛛网膜下腔置入细塑料管，治疗组给予ｂ Ｆ Ｇ Ｆ治疗，对照组同时注入生理盐水。术后切取损伤区脊髓组织进行形态学观察和生化指标测定。结果受损伤段脊髓组织水含量增多，钙离子水平升高，镁离子水平下降，白质内髓鞘结构紊乱，囊性变严重，而ｂ Ｆ Ｇ Ｆ可明显改变上述变化。结论 ｂ Ｆ Ｇ Ｆ对脊髓损伤早期的继发性损害有保护作用。

Objective To study the early protective effects of basic fibroblast growth factor(bFGF) on the experimental acute spinal cord injury.Methods Thirty four SD rats were randomly divided into three groups, and were subjected to contusion of thoracolumber spinal cord. A thin plastic tube was placed in subarachnoid space below the injury level for perfusion. The bFGF treated rats were received 20 μl bFGF(containing bFGF 100 U) at once,30 min,1,2,3,4,6,12,24 and 48 hours after injury, and an equal volume of normal saline was given to the control group at the same time. The injuried spinal cord was detected by morphological observation and biochemical index after injury.Results The degree of ionic disorder in bFGF treated rats was significantly ameliorated and the contents of H 2O were also markedly decreased. The morphological finding showed that the damages of gray and white matter in bFGF treated rats were slighter than those of saline treated rats. Conclusion bFGF has some protective effects on the secondary lesion of early spinal cord injury in rats.