雪旺细胞源神经营养因子预防臂丛神经根性撕脱伤所致 …

RESCUE OF MOTONEURON FROM BRACHIAL PLEXUS NERVE ROOT AVULSION INDUCED CELL DEATH BY SCHWANN CELL DERIVED NEUROTROPHIC FACTOR

目的 研究雪旺细胞源细胞神经营养因子（ＳＤＮＦ）对臂丛神经根性撕脱伤所致前角运动神经元死亡的保护作用。方法 选成年ＳＤ大鼠，制成颈６、７神经根性撕脱伤动物模型，损伤处定期应用ＳＤＮＦ，３周后观察损伤侧脊髓前角运动神经元的成活率和形态学变化，以及一氧化氮合酶（ＮＯＳ）表达的情况。结果 对照组６８．６％的脊髓前角运动神经元死亡，成活神经元胞体明显萎缩，同时表达ＮＯＳ神经元增多；

Objective To study the protective effects of Schwann cell derived neurotrophic factor (SDNF) on motoneurons of spinal anterior horn from spinal root avulsion induced cell death.Methods Twenty SD rats were made the animal model of C 6,7 spinal root avulsion induced motoneuron degeneration,and SDNF was applied at the lesion site of spinal cord once a week. After three weeks, the C 6,7 spinal region was dissected out for motoneuron count, morphological analysis and nitric oxide synthase (NOS) enzyme histochemistry.Results 68.6% motoneurons of spinal anterior horn death were occurred after 3 weeks following surgery, the size of survivors was significantly atrophy and NOS positive neurons increased. However, in animals which received SDNF treatment, the death of motoneurons was significantly decreased, the atro phy of surviving motoneurons was prevented, and expression of NOS was inhibited.Conclusion SDNF can prevent the death of motoneurons following spinal root avulsion.Nitric oxide may play a role in these injury induced motoneuron death.