酸性成纤维细胞生长因子促进引导性骨再生的实验研究

EXPERIMENTAL STUDY ON STIMULATION OF GUIDED BONE REGENERATION BY ACID FIBROBLAST GROWTH FACTOR

目的 研究酸性成纤维细胞生长因子（α Ｆ Ｇ Ｆ）对引导性骨再生（ Ｇ Ｂ Ｒ）的作用，增强 Ｇ Ｂ Ｒ 修复骨缺损的能力。方法 １６ 只新西兰白兔分为四组，每组 ４ 只，造成兔双侧桡骨干１０ ｍ ｍ 节段性骨缺损，以硅胶管桥接骨缺损，实验侧管内置入人基因重组酸性成纤维细胞生长因子（ｈｒａ Ｆ Ｇ Ｆ）２４ μｇ，对侧管内注入生理盐水作对照。于术后２、４、６ 及８ 周各处死一组兔，作 Ｘ 线、大体、组织学观察。结果 实验侧术后２ 周即在骨断端髓腔、骨内膜及皮质断面处有新骨形成，并长入管内血肿，术后４ 周新骨长入血肿中心，８ 周完全骨愈合。对照侧在各阶段新骨形成均不如实验侧，８ 周时仅出现部分骨愈合。结论 酸性成纤维细胞生长因子（ａ Ｆ Ｇ Ｆ）可促进 Ｇ Ｂ Ｒ，增强其修复骨缺损的能力。

Objective To investigate the effect of acid fibroblast growth factor (aFGF) on guided bone regeneration (GBR), to study whether aFGF can promote the repairing ability of GBR in bone defect.Methods 10 mm long segmental defects were created in the diaphyses of both radii in 16 New Zealand rabbits. The defect was bridged with a silicon tube. Human recombinant aFGF was instilled into the tube on the experimental side, while the contralateral tube was instilled with saline as control group.The radiographic, gross and histologic examination of the samples were analyzed at 2,4,6 and 8 weeks after operation.Results On the experimental side, there was new bone formation in the bone medullary cavity,the endosteum and the section surface of the cortex at 2 weeks. At 4 weeks, at the center of the blood clot in the tube there was new bone formation and bone defect was completely healed at 8 weeks. On the control side,new bone formation was less in every period compared with that of the experimental side. At 8 weeks, there was only partial healing of the bone defect.Conclusion It can be concluded that aFGF can promote new bone formation and facilitate GBR in bone defect.