肝素增强血小板生成素刺激巨核细胞生成

Heparin enhances the stimulating effect of thrombopoietin on megakaryocytopoiesis

目的 探索肝素能否增强血小板生成素（ Ｔｐｏ） 刺激巨核细胞生成。方法 以巨核细胞白血病细胞株 Ｍ０７ｅ ，人脐带血 Ｃ Ｄ３ ４ ＋ 细胞和 Ｂ Ａ Ｌ Ｂ／ｃ 小鼠为研究对象，用［３ Ｈ］ Ｔｄ Ｒ 掺入试验，巨核细胞集落（ Ｃ Ｆ Ｕ Ｍ Ｋ） 血浆半固体培养和小鼠体内实验等方法研究肝素或（ 和） Ｔｐｏ 对巨核细胞生成的作用。结果 肝素能增强 Ｔｐｏ 刺激白血病细胞株 Ｍ０７ｅ 增殖、人脐带血 Ｃ Ｄ３ ４ ＋ 细胞衍生的 Ｃ Ｆ Ｕ Ｍ Ｋ 生长和小鼠体内巨核细胞生成。结论 肝素通过增强 Ｔｐｏ 的促巨核细胞生成参与巨核细胞生成的正调控。

Objective To verify whether heparin could enhance the stimulating effect of Tpo on megakaryocytopoiesis. Methods Megakaryocytic leukemia cell line M07e, human cord blood CD 34 + cells and BALB/c mice were used in this study. The effects of heparin and/or Tpo on megakaryocytopoiesis were studied by [ 3H] TdR incorporation assay, CFU MK plasmic semi solid culture and in vivo experiment in BALB/c mice. Results Heparin could enhance the stimulating effect of Tpo on stimulating the proliferation of M07e, the growth of CFU MK from human cord blood CD 34 + cells and in vivo megakaryocytopoiesis in mice. Conclusion Heparin participates in positive regulation of megakaryocytopoiesis by enhancing the megakaryocytopoietic activities of Tpo.