一种新型多吡啶钴(Ⅲ)配合物的合成、表征与DNA的相互作用

Synthesis,Characterization and DNA-Binding Properties of a New Polypyridine Cobalt(Ⅲ) Complex

合成了一种新的多吡啶钴配合物[Co(bpy)2dpapz](ClO4)3.H2O(bpy=2,2′-联吡啶,dpapz=联吡啶并[3,2-a:2′,3′-c]6-氮杂-吩嗪).通过元素分析、红外光谱、紫外光谱和循环伏安法对配合物进行了表征.应用电子吸收光谱、荧光光谱和黏度法研究了配合物与小牛胸腺DNA的作用机理.结果表明,该配合物以插入方式与DNA结合,与DNA的键合常数为1.49×105L.mol-1(50 mmol.L-1NaCl).

A new cobalt（Ⅲ）complex of [Co（bpy）2dpapz]（ClO4）3·H2O（bpy=2,2′-bipyridine,dpapz=dipyrido[3,2-a：2′,3′-c]-6-aza-phenazine） is synthesized,and characterized by elemental analysis,IR,UV and electrochemical methods.The interaction of the complex [Co（bpy）2dpapz]（ClO4）3 with calf thymus DNA has been studied by UV,fluorescence spectra and viscosity measurements.The results suggest that the cobalt complex interacts with DNA by intercalative binding with a binding constant of 1.49×105L·mol-1 at 50 mmol·L-1 NaCl.