湖北省烟粉虱生物型鉴定

The identification of biotypes of Bemisia tabaci in Hubei Province

本研究利用mtDNA COI基因片段作标记,通过序列分析对湖北省烟粉虱Bemisa tabaci(Gennadius)生物型进行了鉴定。结果表明当阳、仙桃、天门、监利、宜昌和武汉6个地理种群烟粉虱的720bp COI基因序列的同源性高达100%,且该6个地理种群烟粉虱与USA-Q型烟粉虱、湖北Q型烟粉虱的同源性也高达100%。枣阳地区烟粉虱与Israel-B型烟粉虱、我国浙江B型烟粉虱、我国湖北B型烟粉虱的同源性为100%。由此可见当阳、仙桃、天门、监利、宜昌和武汉6个地区烟粉虱生物型属于Q型,仅枣阳地区烟粉虱属于B型。

Mitochondrial cytochrome oxidase I（mtDNA COI）gene sequences were utilized to identify biotypes of Bemisa tabaci（Gennadius）in Hubei Province.Of the 720 bp COI sequences examined,those from populations in Dangyang,Xiantao,Tianmen,Jianli,Yichang and Wuhan had as much as 100% similarity.COI sequences of B.tabaci from these six sites were also 100% similar to those of the Q biotype from the USA and Hubei Province.COI sequences of specimens of a B.tabaci population from Zaoyang were 100% similar to specimens of the B biotype from Israel,Zhejiang and Hubei Province.I conclude that B.tabaci from Dangyang,Xiantao,Tianmen,Jianli,Yichang and Wuhan are of the Q biotype where those from Zaoyang are the B biotype.