摘要
acrosome 反应(AR ) ，为精子的一个绝对要求和卵熔化，通过电压依赖者 Ca2+ 隧道和操作店的隧道要求 Ca2+ 的流入进精子。Maitotoxin (MTx ) ，一个动员 Ca2+ 代理人，被显示了是老鼠精子 AR 的有势力 inducer 与类似于带 pellucida (ZP ) 的药理学，可能为两 inducers 建议一条普通小径。使用的 recombinant 人 ZP3 (rhZP3 ) ，老鼠 ZP 和二 MTx 隧道 blockers (U73122 和 U73343 ) ，我们在人和老鼠精子调查了并且比较导致 MTx 、导致 ZP 的艺术。此处，我们报导 MTx 导致了 AR 并且提高了细胞内部的 Ca2+([Ca2+] 我) 在人的精子，哪个被 U73122 和 U73343 堵住。这二混合物也在老鼠精子禁止了导致 MTx 的 AR。在有我们的以前的建议的争论， AR 由 rhZP3 被触发或老鼠 ZP 没被 U73343 堵住，显示在人和老鼠精子，由生理的 ligands 或由 MTx 的 AR 正式就职通过不同小径发生了。U73122，然而并非 U73343 (不活跃的类似物) ，能堵住 phospholipase C (PLC ) 。另一个 PLC 禁止者， edelfosine，也堵住了导致 rhZP3 、导致 ZP 的艺术。这些调查结果在人和老鼠带证实了一条 PLC 依赖的发信号小径的参予导致蛋白质的 AR。尤其是， edelfosine 也禁止了导致 MTx 的老鼠精子 AR 然而并非人的，建议导致毒素的 AR 在老鼠是 PLC 依赖的并且在人 PLC 独立。
The acrosome reaction （AR）, an absolute requirement for spermatozoa and egg fusion, requires the influx of Ca2＋ into the spermatozoa through voltage-dependent Ca2＋ channels and store-operated channels. Maitotoxin （MTx）, a Ca2＋-mobilizing agent, has been shown to be a potent inducer of the mouse sperm AR, with a pharmacology similar to that of the zona pellucida （ZP）, possibly suggesting a common pathway for both inducers. Using recombinant human ZP3 （rhZP3）, mouse ZP and two MTx channel blockers （U73122 and U73343）, we investigated and compared the MTx- and ZP-induced ARs in human and mouse spermatozoa. Herein, we report that MTx induced AR and elevated intracellular Ca2＋ （[Ca2＋]~） in human spermatozoa, both of which were blocked by U73122 and U73343. These two compounds also inhibited the MTx-induced AR in mouse spermatozoa. In disagreement with our previous proposal, the AR triggered by rhZP3 or mouse ZP was not blocked by U73343, indicating that in human and mouse spermatozoa, the AR induction by the physiological ligands or by MTx occurred through distinct pathways. U73122, but not U73343 （inactive analogue）, can block phospholipase C （PLC）. Another PLC inhibitor, edelfosine, also blocked the rhZP3- and ZP-induced ARs. These findings confirmed the participation of a PLC-dependent signalling pathway in human and mouse zona protein-induced AR. Notably, edelfosine also inhibited the MTx-induced mouse sperm AR but not that of the human, suggesting that toxin-induced AR is PLC-dependent in mice and PLC-independent in humans.
