TiO_2喷砂酸蚀处理对人成骨细胞骨保护素和骨保护素配体mRNA表达影响的研究

Effects of TiO_2 sandblast and acid-etched titanium surfaces on mRNA expression of osteoprotegerin and osteoprotegerin ligand in human osteoblast

[目的]探讨纯钛钛片经过喷砂及喷砂酸蚀处理后对人成骨细胞系MG63细胞骨保护素(osteoprotegerin,OPG)及骨保护素配体(osteoprotegerin ligand,OPGL)mRNA表达水平的影响。[方法]纯钛钛片表面分别进行机械打磨、喷砂及喷砂酸蚀处理,将人成骨细胞系MG63细胞接种于钛片表面,采用荧光实时定量PCR法检测OPG、OPGL mRNA表达水平。[结果]MG63细胞在经过喷砂及喷砂酸蚀处理后的钛片上培养后其OPG mRNA水平增高,与机械打磨组相比有统计学意义(P<0.05),而OPGL mRNA表达水平在各组之间没有明显差异(P>0.05)。[结论]经过喷砂及喷砂酸蚀处理的钛片均可促进人成骨细胞表达OPG,从而调节成骨细胞与破骨细胞之间的平衡,促进骨质重建。

[Objective]To investigate the effectiveness of TiO2 sandblasted and acid-etched surfaces of titanium on mRNA expression of osteoprotegerin and osteoprotegerin ligand in human MG63 cells. [Methods]Titanium discs were prepared by machine-polished,sandblasted and acid-etched.MG63 cells were cultured on the titanium surface.The mRNA expressions of OPG and OPGL in MG63 cells were analyzed by real-time PCR.[Results]The level of OPG mRNA was increased in sandblasted group and sandblasted and acid-etched group.There was significant difference（P0.05） between the two groups and the machine-polished group.But the levels of OPGL mRNA were not significantly different（P0.05） among the three groups.[Conclusion]Sandblasted and acid-etched surfaces of titanium could regulate the balance between osteoblast and osteoclast by promoting the expression of OPG in human osteoblast.