昼夜给予逍遥散对慢性肝损伤大鼠的保护作用

Protective effects of administrated Xiaoyao Powder(逍遥散) in the morning and evening on chronic liver injury in rats

目的:研究昼夜不同时间给予逍遥散对CC l4诱发大鼠慢性肝损伤保护作用的差异及其可能机制。方法:采用40%CC l4皮下注射制备大鼠肝损伤模型,分别于早上8点和晚上8点灌胃给予逍遥散,连续45天,观测大鼠一般行为状态,血清ALT和AST、ALB、TP、A/G、T-B IL、HyP,肝组织匀浆SOD、MDA、GSH-PX、ChE,肝组织病理学变化。结果:与模型组比较,逍遥散能显著改善CC l4诱导大鼠慢性肝损伤行为状态、生化指标、肝中纤维组织增生和变性坏死,使结构紊乱的肝细胞趋于正常;同时,ALT、AST、HyP、T-B IL、SOD、GSH-PX和ChE显示昼夜疗效有显著差别,多数指标显示晚间给药和16.52g/kg剂量组的效果更好。结论:逍遥散慢性肝损伤大鼠具有明显的保肝降酶作用,其疗效与给药时间和剂量密切相关。

Objective： To investigate the hepatoprotective effect and its circadian variations of Xiaoyao Powder and the possible mechanisms its protection on chronic liver injury in rats.Methods： The chronic liver injury rat model were induced by subcutaneous injection of 40% CCl4.The rats was given Xiaoyao Powder by gavage in the morning or evening for 45 days.General behavior,the activity of ALT,AST,TP,ALB,A/G,T-BIL,HyP level in serum,SOD,SOD,MDA,ChE,GSH-PX activites in liver homogenate were detected.The pathology of liver was observed.Results： Compared with the model group,the Xiaoyao Powder was found to significantly improve general behavior,reduce the serums levels of ALT、AST、HyP、T-BIL and MDA,increase the activity of SOD,TP,ALB,A/G,GSH-PX and ChE,improve the pathological changes of liver tissue.Meanwhile,Evening administration and 16.52g/kg was displayed a very good effect by most of index.Conclusion ：Xiaoyao Powder show significant protective action on chronic liver injury in rats.The effect of Xiaoyao Powder may be close related to administration time and dose.