甲基阿魏酸在HepG2.2.15细胞培养中对HBV DNA的抑制作用

Inhibitory effect of meth-ferulic acid on the HBV DNA in HepG2.2.15 cells

目的:研究甲基阿魏酸(meth-ferulic acid,MFA)在HepG2.2.15细胞中对HBV DNA合成的抑制作用。方法:采用四甲基噻唑蓝(MTT)法检测MFA对HepG2.2.15细胞的半数毒性浓度(TC50)和最大无毒浓度(TC0);在TC0基础上观察5个不同浓度药物作用于HepG2.2.15细胞,分别在第4d和8d收集细胞培养上清液,采用实时荧光定量PCR法检测上清液HBV DNA的含量。结果:TC0=6.6μg/ml,TC50=179.7μg/ml,MFA对HepG2.2.15细胞毒性较低。无毒浓度下的MFA在HepG2.2.15细胞培养中可有效地减少HBV DNA的拷贝数。结论:MFA在HepG2.2.15细胞培养中能有效的抑制HBV DNA的复制,且毒性较低。

Objective ： To study the inhibitory effect of meth-ferulic acid（MFA） on HBV DNA in HepG2.2.15 cells.Methods ： The TC50 and TC0 of MFA to the HepG2.2.15 cells were determined by MTT.At maximum non-toxic concentration（TC0）,the five different concentrations of MFA were added to HepG2.2.15 cells,which were incubated subsequently.At 4d and 8d after the treatment with drugs,the cell culture supernatant were collected for determining the quantity of HBV DNA detected by real time fluorescence quantitative PCR（FQ-PCR）.Results： TC0 = 6.6 μg/ml,TC50 =179.7μg/ml.MFA had little toxicity to HepG2.2.15 cells.MFA at the maximum non-toxic concentration could decrease the HBV DNA copies in HepG2.2.15 cells.Conclusion ： MFA can inhibit significantly the HBV DNA copies in HepG2.2.15 cells,and is a lower toxic drug.