ANGPTL3在肝细胞癌中的表达及其与肿瘤血管生成的关系

Correlation between ANGPTL3 expression and tumor angiogenesis in hepatocellular carcinoma

目的:研究血管生成素样蛋白3(angiopoietin-like protein3,ANGPTL3)在肝细胞癌(hepato-cellular carcinoma,HCC)中的表达及其与血管生成的关系.方法:应用免疫组织化学方法检测96例肝癌组织和癌旁组织中ANGPTL3和VEGF的表达,同时检测其肝癌组织内微血管密度(microvessel density,MVD).应用Western blot方法检测肝癌组织和癌旁组织中ANGPTL3和VEGF的表达.结果:肝癌组织中ANGPTL3和VEGF表达与癌旁组织中比较显著增加(62.50%vs32.29%;81.25%vs29.17%,P<0.05).HCC组织中存在活跃的血管生成(67.71%vs31.25%,P<0.05),血管生成与ANGPTL3表达相关.Western blot提示肝癌组织中ANGPTL3和VEGF蛋白的表达明显高于癌旁组织,且表达呈正相关.结论:HCC中ANGPTL3高表达是肿瘤血管生成的重要因素.

AIM：To investigate the correlation between the expression of angiopoietin-like protein 3（ANGPTL3）and tumor angiogenesis in hepatocellular carcinoma（HCC）.METHODS：The expression of ANGPTL3 and VEGF proteins in 96 HCC specimens and matched tumor-adjacent specimens was detected by immunohistochemistry and Western blot.Immunohistochemical staining for CD34 was performed in the above specimens to evaluate microvessel density（MVD）.RESULTS：The positive rates of ANGPTL3 and VEGF expression in HCC were significantly higher than that in tumor-adjacent tissue（62.50% vs 32.29%;81.25% vs 29.17%,both P 0.05）.Active angiogenesis was detected in HCC compared to tumor-adjacent tissue（67.71% vs 31.25%,P 0.05）.Tumor angiogenesis was related with ANGPTL3 expression in HCC.The expression of ANGPTL3 and VEGF protein was significantly up-regulated in HCC compared with matched tumor-adjacent noncancerous tissue.CONCLUSION：High expression of ANGPTL3 is associated with tumor angiogenesis in HCC.