SARA在高糖诱导肾小管上皮细胞转分化中的表达变化

SARA expression in high glucose induced epithelium to mesenchymal transition of proximal tubule cells

目的:建立高糖诱导的肾小管上皮细胞转分化模型,检测肾小管上皮细胞转分化过程中Smad锚着蛋白(Smad anchor for receptor activation,SARA)的表达变化。方法:采用Western印迹和实时定量PCR检测细胞波形蛋白(vimentin)、紧密连接蛋白(Zona occludens-1,ZO-1)、SARA蛋白及其mRNA表达水平。结果:30mmol/LD-葡萄糖刺激后,HK-2细胞vimentin蛋白及其mRNA水平呈时间依赖性升高,而ZO-1蛋白及其mRNA水平呈时间依赖性下降,此变化在高糖刺激48h时最为显著。同时,SARA蛋白及其mRNA表达水平呈时间依赖性下降。结论:高糖可诱导HK-2细胞发生转分化,SARA可能作为保护因子参与了这一过程。

Objective To construct the cell model of epithelium to mesenchymal transition of proximal tubule cells induced by high glucose and to determine the expression of Smad anchor for receptor activation （SARA）. Methods Protein expression of vimentin, Zona occludens-1（ZO-1）, and SARA was determined by Western blot, and their mRNA expressions were detected by Real-time PCR. Results After stimulation by 30 mmol/L D-glucose, the protein and mRNA expression levels of vimentin in HK-2 cells increased in a time-dependent manner while the expression of ZO-1 was reduced significantly, especially at 48 h. Meanwhile, SARA was also decreased in a time-dependent manner. Conclusion High glucose can induce renal epithelium to mesenchymal transition, and SARA may be involved in this process as a protector.