摘要
The 4-hydroxy-flavonoid compound luteolin is a phytoestrogen with antioxidant, anti-inflammatory, and anti-tumor properties. The present study analyzed the protective effects of luteolin against rotenone-induced PC12 cell apoptosis in a model of Parkinson＇s disease （PD）. Rotenone （1.6 μmol/L） was utilized to establish the In vitro PD model, resulting in low cell viability and an apoptotic rate of 36.1%. Luteolin （100 μmol/L） pretreatment significantly ameliorated rotenone-induced damage, improved cell morphology and viability, decreased comet tail length, reduced the rate of apoptosis （11.8%）, and down-regulated cleaved caspase-3 expression. Results demonstrate that luteolin protects PC12 cells against rotenone-induced apoptosis by decreasing cleaved caspase-3 expression.
The 4-hydroxy-flavonoid compound luteolin is a phytoestrogen with antioxidant, anti-inflammatory, and anti-tumor properties. The present study analyzed the protective effects of luteolin against rotenone-induced PC12 cell apoptosis in a model of Parkinson＇s disease （PD）. Rotenone （1.6 μmol/L） was utilized to establish the In vitro PD model, resulting in low cell viability and an apoptotic rate of 36.1%. Luteolin （100 μmol/L） pretreatment significantly ameliorated rotenone-induced damage, improved cell morphology and viability, decreased comet tail length, reduced the rate of apoptosis （11.8%）, and down-regulated cleaved caspase-3 expression. Results demonstrate that luteolin protects PC12 cells against rotenone-induced apoptosis by decreasing cleaved caspase-3 expression.
基金
the grant from the Leading Academic Disci-pline Program,National 211 Project of Jinan University (the 3rd phase)
the Science and Technology Project of Guangzhou City,No 2008J1-C181-1
the Fun-damental Research Funds for the Central Universities,No.21609425
