胃黏膜胆色素染色在诊断原发性病理性十二指肠胃反流中的意义

Diagnostic value of porphobilin staining of gastric mucus for primary pathological duodenogastric reflux

目的 探讨胃黏膜胆色素染色对原发性病理性十二指肠胃反流（DGR）的辅助诊断价值.方法 选择2007年1月至2008年4月因上消化道症状就诊的原发性病理性DGR患者纳入原发性病理性DGR组,另选择同期健康志愿者作为正常对照组.对2组患者进行24 h胃内胆汁监测,以胆红素吸收值0.25为界值,取反流时间百分比的中位数（23.60%）作为分界将原发性病理性DGR组进一步分为低反流（反流时间百分比＜23.60%）组和高反流（反流时间百分比≥23.60%）组.所有患者行胃镜检查及胃黏膜活检,对胃黏膜胆色素染色后进行定量分析.结果 原发性病理性DGR组中胃窦、胃角和胃体部黏膜胆色素沉积均明显高于正常对照组（P均＜0.05）.高反流组胃窦部黏膜萎缩及肠上皮化生的检出率均明显高于低反流组（P均＜0.05）,高反流组胃窦、胃角和胃体部黏膜胆色素沉积的腺体数均明显高于低反流组（P均＜0.05）,高反流组胃窦、胃角部黏膜新悉尼系统病理积分均明显高于低反流组（P均＜0.05）.原发性病理性DGR组胃窦部胃黏膜胆色素沉积的腺体数与新悉尼系统病理积分呈明显正相关（r=0.59,P=0.041）,胃角部两因素亦呈明显正相关（r=0.73,P=0.038）,胃体部两因素无明显相关性（r=-0.33,P=0.072）.结论 胃窦部黏膜胆色素染色能够反映胆汁反流程度,并与黏膜损伤程度呈正相关,可有效辅助原发性病理性DGR诊断.

Objective To study the diagnostic value of porphobilin staining of gastric mucus for pfimalt pathologic duodenogastrie reflux （DCR）. Methods A total of 58 DCR patients diagnosed from Janualy, 2007 to April, 2008 were recruited to the study as DGR group, and 21 healthy volunteers as control. All subjects underwent 24-hour intragastric bilimbin monitor and gastroscopy. Bilirubin absorption value of 0. 25 and median reflux time of 23.60% were taken as thresholds to differentiate low reflux group （ reflux time 〈23.60% ） and high reflux group （ reflux time≥23.60% ）. Porphobilin staining of gastric mucosa was quantitatively analyzed. Results Deposition of porphobilin in mucosa of gastric antrum, gastric angle and gastric body in primary pathologi DGR group was significantly higher than those in healthy group （P 〈 0. 05 ）. The occurrence of atrophic and intestinal metaplasia of gastric antrum in high reflux group was significantly higher than that of low reflux group （ P 〈 0. 05 ）. Deposition of porphobilin in mueosa of gastric antrum, gastric angle and gastric body in high reflux group was significantly higher than that of low reflux group （ P 〈 0. 05 ）. The New Sydney system pathological scores of gastric antrum and angle of high reflux group was higher than that of low reflux group （P 〈 0. 05 ）. The deposition uf porphobilin in mueosa of gastric antrum and gastric angle was positively correlated with New Sydney system pathological seores in primary pathological DC, R group （ r = 0. 59, P = 0. 041 and r = 0. 73, P = 0. 038）. Conclusion Porphobilin staining of mucosa in gastric antrum can reflect the severity of bile reflux, and is positively correlated with the extent of gastric mucosal lesion, which may be helpful in diagnosis of primary pathological DGR.