白细胞介素-1基因型与慢性牙周炎、冠心病相关性研究

Interleukin-1 genotype and its assocation with chronic periodontitis and coronary heart disease

目的:探讨白细胞介素-1A+4845(interleukin-1A+4845,IL-1A+4845)、白细胞介素-1B-31(interleukin-1B-31,IL-1B-31)基因型与慢性牙周炎(chronic periodontitis,CP)、冠心病(coronary heart dis-ease,CHD)易感性的关系。方法:收集中重度慢性牙周炎病人127例,冠心病病人123例,慢性牙周炎合并冠心病病人87例和健康对照117例的颊黏膜拭子,提取DNA,用聚合酶链反应-限制性片段长度多态性法(poly-merase chain reaction-restriction fragment length polymorphism,PCR-RFLP)对IL-1A+4845/Fnu 4H1、IL-1B-31/Alu I位点的基因型进行检测,比较各基因型在4组间检出率的差别。结果:①IL-1A+4845基因型在4组间基因型的分布无统计学差异;②IL-1B-31等位基因T在中重度慢性牙周炎组中的检出率显著高于健康对照组,而在冠心病组与健康组、慢性牙周炎合并冠心病组与健康组间的分布无统计学意义。结论:IL-1B-31等位基因T可能是中重度慢性牙周炎的一个遗传学标志。

AIM： To investigate interleukin-1A＋4845 and interleukin-1B-31（IL-1A＋4845.IL-1B-31） genotype and their association with the susceptibility of chronic periodontitis（CP） and coronary heart disease.METHODS： DNA was obtained from buccal swabs of 127 subjects with moderate to severe chronic periodontitis,123 subjects with coronary heart disease,87 subjects with moderate to severe chronic periodontitis and coronary heart disease and 117 matched healthy control individuals.Genotypes of IL-1A＋4845 / Fnu 4H1 and IL-1B-31 /Alu I were analyzed by PCR-RFLP method.Then the differences in distribution of genotype were compared.RESULTS： ①There was no statistical differences about the distribution of IL-1A＋4845 genotype among the four groups.②A significant statistical difference about distribution of IL-1B-31 genotype was found among the four groups.A more significant over-representation of IL-1B-31 allele 2 was found in CP group than in the healthy control individuals.CONCLUSION： The allele of IL-1B-31 may be associated with CP susceptibility.