应用基因功能集群分析方法筛选创伤性深静脉血栓形成的关键基因

Gene function assembly analysis to screen key genes associated with deep vein thrombosis after trauma

目的 应用基因功能集群分析方法筛选创伤性深静脉血栓形成（DVT）的关键基因,探讨这些基因在创伤性DVT发生中的意义. 方法选取30只SD大鼠,随机分为对照组（A组,10只）和模型组（B组,20只）,通过定量打击方法建立创伤性DVT的动物模型.模型组造模后5 d根据血栓形成情况再分为2个亚组（每组10只）：血栓形成组（B1组）和无血栓形成组（B2组）.采用Genechip Rat Genome 430 2.0基因表达谱芯片检测DVT发生、发展过程中创伤局部静脉内基因差异表达的变化.采用基因功能集群分析方法对B1组与B2组差异表达基因进行重点分析,寻找其中导致血栓形成的生物学状态最显著、最集中的基因功能. 结果血栓形成组与无血栓形成组主要涉及补体激活、细胞发生、生长、运动及代谢等功能,筛选出的关键基因主要有B因子、补体4结合蛋白α、纤溶酶原激活剂抑制物1及尿激酶型纤溶酶原活化剂受体等.血栓形成组上述关键基因的丰度表达值分别为1.6、-0.2、2.1、5.1,无血栓形成组其丰度表达值分别为-0.5、-1.4、2.7、3.3. 结论在创伤性DVT的演化过程中,B因子、补体4结合蛋白α、纤溶酶原激活剂抑制物1及尿激酶型纤溶酶原活化剂受体等基因是影响血栓形成与否的关键基因.

Objective To screen key genes related to deep vein thrombosis （TDVT） after trauma using gene function assembly analysis. Methods Thirty Sprague Dawley rats were randomized into control, thrombosis and non-thrombosis groups. Traumatic limb DVT models were established in rats through quantitative beating on the bilateral posterior limbs. The Genechip Rat genome 430 2. 0 genechips were applied to detect changes in genes expressions on difference phases of DVT. On the basis of the differential gene expressions in the thrombosis and non-thrombosis groups, the gene function assembly analysis was conducted to define the most significant and concentrated gene functions leading to the biological characters of DVT.Results B factor （bf）, complement 4 binding protein α （C4bpα）, plasminogen activator inhibitor 1 （serpinel）, urokinase-type plasminogen activator receptor （plaur） were screened to be the key genes related to DVT, because they were found to be involved in the functions like complement activation, development,growth, morphogenesis, primary metabolism, cell motility, protein metabolism, localization of cell, locomotion and localization. The abundance values of the genes expressed were 1.6, -0. 2, 2. 1, 5. 1 in the thrombosis group, and -0. 5, - 1.4, 2. 7, 3. 3 in the non-thrombosis group. Conclusion Bf, C4bpα,serpinel, plaur may be the key genes that play a role in the process of DVT.