医用透明质酸钠自组装形貌的纳米探测

Nanometer detection of self-assembly morphology of sodium hyaluronate injection on mica

目的采用原子力显微镜)对医用透明质酸钠的自组装在纳米水平上进行探测。方法采用原子力显微镜在自然条件下对不同浓度医用透明质酸钠在云母表面的自组装体系进行纳米分辨率成像,分析及比较。结果医用透明质酸钠浓度为0.001mg/ml时在云母表面自组装成花斑状结构,0.01mg/ml时自组装成不规则的珍珠式网状结构,浓度为0.1mg/ml时自组装成树枝形分形结构,当浓度为1mg/ml及5mg/ml时则在云母表面形成均匀的杆状结构及球状微粒结构。5mg/ml透明质酸钠在云母表面形成的球状微粒的粒径及高度(粒径为197.97±78.48nm,高度30.79±18.67nm)较浓度为0.1mg/ml透明质酸钠微粒的粒径及高度(粒径为49.52±11.93nm,高度5.37±1.59nm)明显增大(P<0.05)。结论不同浓度医用透明质酸钠在云母表面的自组装形貌不同,致密均匀的球状微粒、花斑状及网状形貌对于研究其在体内发挥的生理作用具有重要意义,树枝状结构符合分形学中的有限扩散凝聚模型。

Objective To detect the self-assembly morphology of sodium hyaluronate injection on mica using atomic force microscopy （AFM）. Methods Atomic force microscopy with nanometer resolution was used to observe the self-assembly morphology of different concentrations of sodium hyaluronate injection on mica at room temperature. Results The self-assembly morphology of 0.001,0.01,and 0.1 mg/ml sodium hyaluronate injection on mica featured piebald,reticular and dendritic structures,respectively. At 1 and 5 mg/ml,sodium hyaluronate injection displayed bacilliform and spherical structures on mica,respectively; the diameter and height of the particles of 5 mg/ml sodium hyaluronate was 197.97±78.48 nm and 30.79±18.67 nm,significantly greater than those of 0.1 mg/ml sodium hyaluronate injection （49.52±11.93 nm and 5.37± 1.59 nm,respectively,P0.05）. Conclusion The self-assembly morphology of sodium hyaluronate injection on mica varies with its concentration. The piebald and reticular structure may facilitate the function of sodium hyaluronate,and the dendritic feature resembles the representative model of diffusion-limited aggregation （DLA）.