姜黄素对肝纤维化大鼠肝组织Bcl-2及Bax表达的影响

Effect of Curcumin on Expression of Bcl-2 and Bax in Hepatic Tissue of Hepatic Fibrosis Rats

目的:观察姜黄素对肝纤维化大鼠肝组织抑凋亡细胞(Bcl-2)、促凋亡细胞(Bax)的影响,探讨其抗肝纤维化的机理。方法:采用四氯化碳皮下注射加乙醇灌胃制备大鼠肝纤维化模型,用免疫组织化学方法(SABC)检测各组大鼠肝组织中Bcl-2、Bax的含量。结果:与模型组比较,姜黄素大、中剂量组肝组织纤维间隔中Bcl-2的表达明显下降(P<0.05),Bax的表达无明显改变(P>0.05),Bax/Bcl-2比值明显升高(P<0.05)。结论:姜黄素可有效升高纤维间隔中Bax/Bcl-2比值,并能抑制肝星状细胞(HSC)活化增殖,诱导活化的HSC凋亡,具有治疗肝纤维化的作用。在一定有效剂量范围内,其抗肝纤维化作用强度与剂量无明显依赖性。

Objective：Observe the effect of Curcumin on Bcl-2 and Bax of hepatic fibrosis tissues in rats,to further discuss the mechanism of antifibrotic action.Methods：Rat models of hepatic fibrosis were established by hypodermic injection of Carbon tetrachloride and filling the stomach with alcohol.The content of Bcl-2 and Bax in liver tissue of rats were detected by immunohistochemical technique（SABC）.Result：Compared with model group,the Bcl-2 expression drops obviously in the liver fiber gap of middle and big dosage group of Curcumin（P0.05）,the Bax expression changes indistinctively（P0.05）,the ratio of Bax/ Bcl-2 in fiber gap was decreased by large（P0.05）.Conclusions：Curcumin can decreased the ratio of Bax/ Bcl-2 in fiber gap,then suppressed the HSC activation and multiply,and inducted activated HSC to apoptosis.The Curcumin has the function of treatment hepatic fibrosis,the relevance didn＇t present between anti-hepatic fibrosis function and the dosage.