酸性氧化电位水对大肠杆菌杀灭效果及其主导因素研究

RESEARCH ON THE DISINFECTION EFFECT AND LEADING FACTORS OF ELECTROLYZED OXIDIZING WATER ON Escherichia coli

目的研究酸性氧化电位水杀灭大肠杆菌的效果及其主要影响因素。方法采用悬液定量杀菌试验和理化分析方法以及光学显微镜观察,进行了实验室研究。结果在清洁条件下,用含有效氯33.1 mg/L的EOW作用0.5 min,对悬液内大肠杆菌达到完全杀灭。菌悬液与含30 g/L小牛血清白蛋白溶液对倍稀释,明显影响EOW的杀菌效果。单因子低pH值或单独提高有效氯,其杀菌效果均明显低于相同浓度的EOW。经碘化丙锭染色和显微镜观察显示,EOW作用后的大肠杆菌细胞膜受到严重破坏。结论 EOW的杀菌作用是以有效氯为主导,低pH值及高ORP值为促进作用的三者协同作用的结果。

Objective To study the disinfection effect of electrolyzed oxidizing water（EOW） on Escherichia coli and the main influencing factors in its germicidal efficacy. Method Suspension quantitative germicidal test,physicochemical analysis methods and fluorescence microscopy were carried out to study EOW＇s disinfection effect and the influencing factors. Result EOW with available chlorine concentration（ACC） of 33.1 mg/L could kill E.Coli completely in 0.5 min.The disinfection effect was obviously inhibited when the bacteria suspension was diluted in 1：1 with 30 g/L bovine serum albumin（BSA）.When pH was decreased or ACC was increased alone,the disinfection effects were much lower than that of EOW.After being stained by propidium iodide（PI）,the cell membrane of E.coli could be proved seriously damaged in fluorescent microscopy. Conclusion EOW＇s high disinfection ability is caused by a synergistic effect of different factors,in which ACC plays the leading role and low pH and high ORP are important promotion.