载体回收菌量影响因素的试验观察

EXPERIMENTAL OBSERVATION ON INFLUENCIAL FACTORS OF RECOVERED BACTERIAL AMOUNT ON CARRIER

目的研究影响染菌载体上回收菌量的因素,为载体定量杀菌试验提供依据。方法采用定量染菌和活菌计数的方法,对载体种类、细菌种类和干燥时间等因素的影响进行了观察。结果滴染在不锈钢载片和玻璃载片上的细菌芽孢平均回收率分别为34.23%和37.20%;纸片上细菌芽孢平均回收率仅为13.39%。5种细菌滴染在布片上,平均回收率没有明显差异。滴染的染菌载体在37℃干燥20 min以上,对细菌回收率有明显影响。结论染菌载体性质和干燥时间对回收菌量有明显影响,不同细菌种类在相同载体上回收率无明显差别。

Objective To observe influential factors of recovered bacterial amount on carrier and provide the basis for carrier quantitative germicidal test. Methods Quantitative bacteria dyeing and bacteria counting method were used to observe the influences of carrier type,bacteria type and drying time. Results The average recovered rate of bacteria spores on the stainless steel slide and glass slide were 34.23% and 37.20% respectively;that of spores on paper slide was 13.39%.There were no significant differences on bacteria recovered rates of 5 kinds of bacteria on fabric slides.There was significant influence on bacteria recovered rate when bacteria carrier dried at 37℃ for over 20 min. Conclusion The kinds of bacterial carrier and drying time have strong influences on recovered bacteria count and different kinds of bacteria on the same slide have no significant differences.