蓝玉簪龙胆提取液抗呼吸道合胞病毒的初步实验研究

Empirical Study of Anti-RSV Effect on Gentiana Veitchiorum

目的:观察蓝玉簪龙胆水煎液及正丁醇、乙酸乙酯、氯仿提取液在体内外的抗呼吸道合胞病毒(RSV)作用。方法:通过观察细胞病变效应(CPE)和MTT比色,评价不同浓度的蓝玉簪龙胆提取液抑制RSV感染HEP-2细胞的病变作用;通过HE染色和免疫荧光染色,评价蓝玉簪龙胆水煎液对RSV感染小鼠肺组织炎性病变抑制效果。结果:体外实验结果与病毒对照组相比,蓝玉簪龙胆水煎液及正丁醇、乙酸乙酯、氯仿提取液分别最高可使RSV感染细胞后活力升至77.3%±7.1%5、8.4%±6.5%、50.4%±3.8%、47.5%±5.2%;体内实验结果与病毒对照组相比,蓝玉簪龙胆水煎液治疗组炎症反应明显轻于病毒对照组;免疫荧光染色示RSV感染小鼠成功。结论:蓝玉簪龙胆不同浓度提取液均可抑制RSV在细胞和动物模型所致病变。

Objective： To observe the anti-RSV effect of Gentiana veitchiorum decoction,n-butanol extract,ethyl acetate extract,chloroform extract in vitro and vivo.Methods： To observe cytopathic effect（CPE） and Methyl thiazolyl tetrazolium（MTT） assay,the effect of anti-RSV was evaluated with RSV infected hep-2 cells for different Gentiana veitchiorum.The degree of mouse lung tissue inflammation was evaluated by experimental hematoxylin and eosin（HE） staining and immunofluorescence.Results： After the different Gentiana veitchiorum treatment,the cell viability rose as high as 77.3%±7.1%,58.4%±6.5%,50.4%±3.8% and 47.5%±5.2%.In animal models,the lung inflammatory response in the Gentiana veitchiorum decoction treated group were lower than that of the control group of viral model by HE.Immunofluorescence method confirmed that the infected mouse models of RSV were successful.Conclusions： The different Gentiana veitchiorum extracting solution in different concentrations can restrain cytopathic changes.