依达拉奉对大鼠实验性自身免疫性脑脊髓炎的保护作用

Protective Effect of Edaravone on Experimental Autoimmune Encephalomyelitis in Rats

目的:研究依达拉奉对实验性自身免疫性脑脊髓炎(Experimental Autoimmune Encephalomyelitis,EAE)的影响。方法:72只健康成年雌性Wistar大鼠随机分为:正常对照组、EAE组、EAE+小剂量依达拉奉组、EAE+大剂量依达拉奉组(n=18)。正常对照组注射生理盐水,其它组采用自制完全抗原诱导EAE模型。EAE组建模后不做任何处理,EAE+小剂量依达拉奉组、EAE+大剂量依达拉奉组分别在建模后给予依达拉奉4mg/k·d、10mg/k·d。比较各组发病率并行神经功能评分,取脊髓组织行HE染色、iNOS、OPN免疫组织化学染色观察。结果:依达拉奉干预组大鼠较EAE组发病率、神经功能缺损评分均明显降低(P<0.05)。HE结果显示依达拉奉干预组较EAE组炎症反应减少,损伤程度减轻。依达拉奉组iNOS、OPN表达均明显小于EAE组(P<0.05)。大剂量依达拉奉iNOS、OPN表达均低于小剂量依达拉奉组(P<0.05)。结论:依达拉奉对EAE具有保护作用,可能与抑制小神经胶质细胞活化,减轻炎症反应,降低iNOS和OPN表达有关。

Objective：To study the effect of edaravone on experimental autoimmune encephalomyelitis（EAE） in rats.Methods：72 adult healthy female Wistar rats were randomly divided into 4 groups：normal control group,EAE group,EAE group plus low dose edar-avone group,EAE group plus large dose of edaravone group（n = 18）.Normal group were subcutaneously injected saline water.The EAE model were established with self-made complete antigen,then the small（4mg/kg o d） and large（10mg/kg o d） doses of edaravone were given according the experimental protocol.The incidence and the scores of neurologic impairment were recorded,also the HE staining,the inducible nitro oxide synthase（iNOS） and Osteopontin（OPN） immunohistochemistry staining were performed and analyzed.Results：After the treatment of edaravon,the incidence and scores of neurologic impairment were significantly decreased（P0.05）.HE staining showed that the edaravone group with alleviation of inflammatory reaction and degree of injury.Either low or large-dose edaravone group,the expression of iNOS,OPN was significantly less than the EAE group（P0.05）.In large-dose edaravone group,the expres-sion of iNOS and OPN was significantly lower than low-dose edaravone group（P0.05）.Conclusion：The results suggested that Edar-avone have protective role on EAE,which maybe related to the inhibition of microgliacyte activation,alleviation of inflammatory reac-tion and reduction of iNOS and OPN expression.