单链抗体的研究进展

Progress in Single-Chain Antibody

单链抗体即单链抗体可变区片段(single-chain antibody variable fragment,or ScFv)是由抗体重链可变区和轻链可变区通过一段10-25个氨基酸的连接肽连接而成,其分子质量小,穿透力强,特异性好,免疫原性低,在免疫学和医学方面得到了广泛应用。本文就单链抗体的结构设计、展示系统、表达和应用方面做一综述。

The single-chain antibody（single-chain antibody variable fragment,or ScFv） is made up of the variable region of the heavy and light chains,which are connected together by a polypeptide linker of typically 10-25 amino acid residues in length.The advan-tages of ScFv are the small size of molecular mass,higher affinity for target antigens,and imposing minimal antigenicity in recipient hosts.So the single-chain antibody had been widely applied in immunology and medical field.In this paper,the molecular design of sin-gle-chain antibodies,display systems,expression,and applications were reviewed.