百脉根结瘤信号通道蛋白NSP1和NSP2的相互作用

Interaction of Lotus japonicus NSP1 and NSP2

豆科植物百脉根是研究共生固氮体系的模式植物之一,对其根瘤菌共生信号转导途径和作用机制的研究,有助于揭示根瘤菌与豆科植物共生关系建立和共生体形成的奥秘。本研究利用酵母双杂交系统和体外蛋白质相互作用技术,证明百脉根GARS类家族转录因子结瘤信号通道蛋白NSP1和NSP2在体外体内均可以相互作用,并将相互作用的位点定位在NSP1的GRAS结构域的LHRII区域和NSP2的LHRI区内。

Lotus japonicus in legumes is one of the model plants in the study of the system on the symbiotic nitrogen fixation.The research of the symbiotic signaling pathway and the mechanism of interaction of rhizobial bacteria helps to discover the secret about the establishment of symbiotic relationship and the formation of the symbiont between rhizobial bacteria with legumes.In this study,the interaction of the two GRAS family proteins,nodulation signaling pathway1（NSP1） and nodulation signaling pathway2（NSP2）,was confirmed in vivo and vitro using a yeast two-hybrid system and protein-protein interaction in vitro.The interacting loci were assessed to be the LHRI domain of NSP2 and LHRII domain of NSP1.