电麻醉对西伯利亚鲟幼鱼血液生化指标的影响

Effects of electronarcosis on haematological biochemistry of juvenile Acipenser baerii

设置5个麻醉组（15-35 V）与1个对照组（0 V）,研究直流电麻醉对西伯利亚鲟幼鱼血液13项生化指标的影响。随着电压强度升高,西伯利亚鲟麻醉组总蛋白与白蛋白质量浓度逐渐升高,35 V组总蛋白质量浓度显著高于15 V组与20 V组。高密度脂蛋白、低密度脂蛋白和甘油三酯浓度在15 V组中最低,在35 V组最高,35 V组显著高于对照组与15 V组。对照组总胆固醇浓度为（2.35±0.69）mmol/L,15 V组与35 V组之间具有显著差异。对照组血糖浓度为（2.21±0.30）mmol/L,低于各麻醉组的浓度。尿素与总胆红素浓度在15 V组最低,35 V组最高。谷丙转氨酶、谷草转氨酶与乳酸脱氢酶活性相对稳定,对照组与各麻醉组间均无显著性差异。对照组血清碱性磷酸酶活性为（218.33±67.10）U/L,35 V组活性显著高于对照组与15 V组。综合各项血液生化指标表明,直流电麻醉是种较好的麻醉方法,西伯利亚鲟幼鱼适宜电麻醉强度为20-30 V。

Five electronarcosis groups and one control group were designed to study effects of electronarcosis on haematological biochemistry of juvenile Siberian sturgeon Acipebser baerii.Results showed that total protein and albumin of sturgeon in the electronarcosis groups increased positively with voltage intensity.The values of total protein in the 35 V group were significantly higher than those in the 15 V and 20 V groups.The concentrations of high density lipoprotein cholesterin,low density lipoprotein cholesterin and triglycerides were the lowest in the 35 V group and was the highest in the 15 V group.These three indexes in the 35 V group were significantly higher than those in the control group and the 15 V group.There was significant difference between the 15 V group and the 35 V group.The value of glucose in the control group was 2.21 mmol/L.The values of glucose in each electronarcosis group were higher than those shown in the control group.Urea and total bilirubin were the lowest in the 15 V group and the highest in the 35 V group.The values of alanine aminotransferase,aspartate aminotransferase and lactate dehydrogenase were relatively steady.The value of alkaline phosphatase in the control group was 218.33 U/L.The value of alkaline phosphatase in the 35 V group was significantly higher than that in the control group and the 15 V group.The present study showed that electronarcosis was a good anaesthesia method.The appropriate voltage of electronarcosis in Siberian sturgeon was from 20 to 30 V.