白头翁诱导胃癌细胞凋亡的研究

Study of Apoptosis in Gastric Cancer Cells Induced by Chinese Pulsatilla Root

目的研究中药白头翁对诱导胃癌BGC823细胞株凋亡过程的影响作用,探讨白头翁抗肿瘤的可能机制。方法以体外培养的胃癌BGC823细胞株为研究对象,MTT法检测不同浓度白头翁对BGC823细胞活性的影响,采用流式细胞术检测不同浓度白头翁作用胃癌BGC823细胞24h后细胞凋亡率。结果 白头翁对BGC823细胞活性的抑制呈浓度依赖性,白头翁抑制BGC823细胞增殖可能由于其诱导胃癌细胞凋亡所致。结论白头翁能诱导胃癌BGC823细胞株发生细胞凋亡,有望成为胃癌的辅助治疗药物。

Objective To study the influence on apoptosis in gastric cancer cell lines BGC823 induced by Chinese Pulsatilla root, and explore the possible mechanism of antitumor in Pulsatilla. Methods Take the gastric cancer BGC823 cell in vitro as the study object,the active of BGC823 cell with different concentrations of Pulsatilla is examined by MTI＂ assay,and BGC823 cell apoptosis examined by flow cytometry while different concentrations of Pulsatilla after BGC823 cell was processed 24 hours. Results The active of BGC823 cell is inhibited by Pulsa^illa on the concentration dependent inhibition,this role of inhibition is due to BGC823 cell apoptosis was induced by Pulsatilla. Conclusion Chinese Pulsatilla root can induce cancer cell apoptosis,BGC823 cell should be the basis of clinical medicine for cancer treatment.