八角莲的化学成分研究

Chemical constituents from Dysosma versipellis

目的研究八角莲Dysosma versipellis根茎的化学成分。方法运用硅胶、凝胶及RP-HPLC等多种色谱技术对八角莲根茎的化学成分进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构。结果从八角莲根茎95%乙醇提取物的醋酸乙酯和正丁醇萃取部位分离并鉴定了14个化合物,其中木脂素类化合物10个,分别为4′,5′-二去甲基鬼臼毒素(4′,5′-didemethylpodophyllotoxin,1)、鬼臼毒素(podophyllotoxin,2)、4′-去甲基鬼臼毒素(4′-demethylpodophyllotoxin,3)、山荷叶素(diphyllin,4)、鬼臼毒酮(podophyllotoxone,5)、苦鬼臼毒素葡萄糖苷(picropodophyllotoxin-4-O-β-D-glucoside,6)、4′-去甲基鬼臼毒素葡萄糖苷(4′-demethylpodophyllotoxin-4-O-β-D-glucoside,7)、山荷叶素葡萄糖苷(diphyllin-4-O-β-D-glucoside,8)、鬼臼毒素葡萄糖苷(podophyllotoxin-4-O-β-D-glucoside,9)、八角莲醇(dysosmarol,10);黄酮类化合物3个,分别为山柰酚(kaempferol,11)、槲皮素(quercetin,12)、山柰黄素-3-O-β-D-吡喃葡萄糖苷(kaempferol-3-O-β-D-glucoside,13);甾醇类化合物1个,为β-谷甾醇(β-sitosterol,14)。结论化合物1为新天然产物,化合物7和8为首次从该属植物中分离得到。

Objective To study the chemical constitutents from the roots and stems of Dysosma versipellis.Methods Silica gel column,Sephadex LH-20,and reverse phase HPLC chromatography were used to isolate the chemical constituents,and the structures of the isolated compounds were elucidated by spectral analysis and physicochemical properties.Results Fourteen compounds were isolated from EtOAc and n-butanol fraction of the 95% ethanol extract in the roots and stems of D.versipellis,including 10 lignans,i.e.4′,5′-didemethylpodophyllotoxin（1）,podophyllotoxin（2）,4′-demethylpodophyllotoxin（3）,diphyllin（4）,podophyllo-toxone（5）,picropodophyll-otoxin-4-O-β-D-glucoside（6）,4′-demethylpodophyllotoxin-4-O-glucoside（7）,diphyllin-4-O-β-D-glucoside（8）,podophyllotoxin-4-O-β-D-glucoside（9）,and dysosmarol（10）;three flavonoids,i.e.kaempferol（11）,quercetin（12）,and kaempferol-3-O-β-D-glucoside（13）;and one sterol,i.e.β-sitosterol（14）.Conclusion Compound 1 is a new natural product,and compounds 7 and 8 are isolated from the plants of Dysosma Woodson for the first time.