冰片及石菖蒲促进羟基红花黄色素A透过血脑屏障的实验研究

Enhancing effect of Borneolum Syntheticum and Acori Talarinowii Rhizoma on penetrating blood-brain barrier of hydroxysafflor yellow A

目的考察芳香开窍药冰片及石菖蒲对羟基红花黄色素A(HSYA)血脑屏障(BBB)通透性的影响。方法 ig给予大鼠20.00 mg/kg HSYA混悬液,分别配伍冰片及石菖蒲水提液,给药后采集大鼠血浆及脑组织。HPLC测定大鼠血浆及脑组织内HSYA浓度,以各组HSYA的AUC脑/AUC血为评价指标,进行冰片及石菖蒲对BBB通透性影响的评价。结果 HSYA、HYSA+冰片、HYSA+石菖蒲组HSYA在大鼠血浆及脑匀浆中的AUC0～8 h分别为(77 228.76±2 873.19)、(81 949.04±2 283.11)、(28 479.63±2 431.71)ng.mL-1.min及(55 925.0±2 434.28)、(82 768.53±3 277.00)、(70 914.29±2 900.71)ng.mL-1.min,各组AUC脑/AUC血分别为0.72、1.01、2.49。结论 HSYA配伍冰片及石菖蒲后,AUC脑/AUC血均显著升高(P<0.05)。冰片及石菖蒲均对BBB有开启作用,且石菖蒲可影响HSYA在大鼠体内的分布。

Objective To investigate the enhancing effect of Borneolum Syntheticum and Acori Talarinowii Rhizoma on penetrating blood-brain barrier（BBB） of hydroxysafflor yellow A（HSYA） in rat.Methods The concentration of HSYA in rat plasma and brain was determinated after ig administration of 20.00 mg/kg of HSYA to rats with or without Borneolum Syntheticum and Acori Talarinowii Rhizoma.And then,the character of penetrating BBB through the result of the AUCbrain/AUCbloodwas evaluated.Results The AUC0—8 h were（77 228.76 ± 2 873.19）,（81 949.04 ± 2 283.11）,and（28 479.63 ± 2 431.71） ng.mL-1.min for blood and（55 925.0 ± 2 434.28）,（82 768.53 ± 3 277.00）,and（70 914.29 ± 2 900.71） ng.mL-1.min for brain of the control group,Borneolum Syntheticum group,and Acori Talarinowii Rhizoma group.The AUCbrain/AUCblood were 0.72,1.01,and 2.49,respectively.Conclusion Borneolum Syntheticum and Acori Talarinowii Rhizoma do enhance the penetrating BBB of HSYA（P0.05）.Further more,Acori Talarinowii Rhizoma could affect the distribution of HSYA in rats.