短葶山麦冬遗传多样性SRAP标记研究

Genetic diversity of Liriope muscari using SRAP markers

目的对短葶山麦冬进行遗传多样性分析。方法利用SRAP分子标记技术对47份短葶山麦冬材料进行遗传多样性分析。结果 15对引物共扩增出323条多态性带,平均多态位点百分率为88.47%。平均引物多态信息量(PIC)为0.90。Nei’s基因多样性指数(H)为0.197 7,Shannon’s信息指数(I)为0.319 0。遗传分化系数(Gst)为0.252 8。UPGMA聚类表明遗传相似系数为0.59～0.99。结论短葶山麦冬遗传多样性水平较高,遗传变异主要在居群内。

Objective To study the genetic diversity of Liriope muscari.Methods The genetic diversity of 47 populations was analyzed by SRAP marker technique.Results Fifteen primers amplified 323 polymorphic bands,and the average percentage of polymorphic bands reached to 88.47%.The average of polymorphism information content（PIC） was 0.90.Nei’s gene diversity index（H） was 0.197 7 and Shannon’s information index（I） was 0.319 0.Gene differentiation index（Gst） was 0.252 8.Cluster analysis using UPGMA method showed that genetic similarity coefficient ranged in 0.59—0.99.Conclusion L.muscari shows higher genetic diversity and the majority of genetic variation occurs in populations.