1株近平滑假丝酵母的分离及其鉴定

Isolation,Purification and Identification of a Candida parapsilosis strain

采用平板稀释法从土壤及水果中分离出1株近平滑假丝酵母（Candida parapsilosis）,YPD培养基培养酵母,利用分子生物学方法对其rRNA基因内转录间区（ITS区）进行了克隆测序,并与GenBank中已有的有关序列进行比较及系统发育分析。测序结果表明该序列长度为547 bp,与GenBank中近平滑假丝酵母同源率在98.5%~100%之间,进化分析表明与C.parapsilosis（EF193067）、C.parapsilosis UOA/HCPF（FJ872013）属于一单独分支中,形态结果及分子鉴定表明近平滑假丝酵母培养成功,为其进一步开发利用提供了依据。

A Candida parapsilosis（Cp） strain was isolated using YPD medium plate dilution method from soil and fruits,identified by molecular biological method with rRNA gene cloning and sequencing in its ITS transcription domain;and made the comparison with relevant sequence available in Genbank and phylogenetic analysis.The results of the sequencing showed that homologous rate of the Cp strain was 98.5%~100% with the one in Genbank.Evolution analysis showed that it belonged to independent branch of Cp（EF193067） and Cp UOA/HCPF（FJ872013）.Its morphology and molecular identification indicated that the Cp strain was successfully cultivated and this provided a foundation for its further development and utilization.