高效液相色谱法对复合食品包装袋中三唑类光稳定剂含量的测定

Determination of Triazole Ultraviolet Stabilizer in Composite Laminated Food Packaging Bags by High Performance Liquid Chromatography

以复合食品包装袋为实验材料,首次建立了一种用高效液相色谱法(HPLC)同时检测复合食品包装袋中2-(2'-羟基-3'-叔丁基-5'-甲基苯基)-5-氯代苯并三唑(UV-326)和2-(2'-羟基-3',5'-二叔丁基苯基)-5-氯代苯并三唑(UV-327)含量的分析方法。实验采用超声波辅助提取,提取液旋转蒸发后,经固相萃取柱净化,氮吹至干,甲醇定容,高效液相色谱仪进行分析,外标法定量。该方法对复合食品包装袋中UV-326和UV-327的定量下限均为0.2 mg/kg,线性范围均为0.2～10 mg/kg,加标回收率分别为75%～90%、80%～85%,相对标准偏差分别为3.6%～6.7%、3.4%～4.2%。方法可满足复合食品包装袋中UV-326和UV-327的检测要求。

A new high performance liquid chromatographic （HPLC） method with ultraviolet detection was firstly developed for the determination of 2-（3-tert-butyl-2-hydroxy-5-methylphenyl）-5-chloro-2h- benzotriazole （ UV-326 ） and 2-（ 2-hydroxy-3, 5-di-tert-butylphenyl ） -5-chlorobenzotriazole （ UV-327 ） in composite laminated food packaging bags. The samples were extracted by ultrasound assisted extraction method. Then, the extracts were further concentrated by rotary evaporation technology and cleaned up with solid phase extraction column. The eluent was evaporated to near dryness under a stream of nitrogen and redissolved with methanol. The separation of target compounds was performed with Agilent ZORBZX SB-Cjs column（250 mm ×4.6 mm, 5 μm） using methanol as elution reagent at a flow rate of 1.0 mL/min at 30℃. The quantitative analysis of target compounds was performed by the external standard method at a detection wavelength of 310 nm. The result indicated that the calibration curves were linear in the range of 0. 2 - 10 mg/kg for UV-326 and UV-327 with both limits of quantitation of 0. 2 mg/kg. The average recoveries of UV-326 and UV-327 were in the range of 75% -90% and 80% -85% , respectively, with RSDs of 3.6% -6.7% and 3.4% -4.2% , respectively. The method could be applied in the determination of UV-326 and UV-327 in composite laminated food packaging bags.