壳寡糖通过降低线粒体膜电位促进A549细胞凋亡

Chitooligosaccharides induces apoptosis of A549 cells through decline of mitochondrial membrane potential

壳寡糖(chitooligosaccharides,COS)分子量小,可溶性好,具有抗肿瘤等多种生物活性。以人肺腺癌A549细胞系为研究对象,探究COS对A549细胞线粒体膜电位、细胞生长与死亡的影响。MTT结果表明COS浓度越高,刺激时间越长,其对A549细胞生长的抑制作用越明显;流式细胞仪检测结果显示,1mg/mL的COS明显地降低了细胞线粒体膜电位水平;活死细胞荧光染色实验表明,COS组死亡细胞比率提高,与对照组相比具有显著性差异。结果提示,COS可能通过降低线粒体的膜电位而促进A549细胞凋亡。

Chitooligosaccharides（COS） has been reported to have a lower molecular weight and a higher water solubility,and has lots of properties including antitumor activity.In this study,we investigated the role of COS on the mitochondrial membrane potential,proliferation and apoptosis of A549 cells.MTT results showed that COS inhibited A549 cell proliferation with increase of COS concentration and inducing time.1mg/mL COS significantly declined the mitochondrial membrane potential,which was examined by flow cytometry.dead-live cell staining results showed that after incubating with COS,the red dead cells increased compared with control group.The results suggested that COS regulated apoptosis of A549 cells through decline of mitochondrial membrane potential.