冷应激对雏鸡肠组织中HSP70 mRNA表达的影响

Effects of cold stress on HSP70 mRNA expression in intestines of chickens

探讨冷应激对雏鸡肠道中热休克蛋白70(HSP70)mRNA表达量的影响。120只1日龄的伊莎公鸡,饲养至15日龄,随机平均分为12组,在(12±1)℃进行急性和慢性冷应激处理。急性冷应激时间为0,1,3,6,12和24h;慢性冷应激时间为5、10和20d,并各设对照组。各时间点随机剖杀5只,取十二指肠、空肠、回肠和盲肠用实时定量PCR法检测HSP70 mRNA的表达。结果表明,急性应激时,各组织中HSP70 mRNA的表达整体降低,但在初期显著升高(P<0.05)。慢性冷应激时,各组织中HSP70 mRNA整体显著升高(P<0.05)。结果显示,冷应激可以改变雏鸡肠组织中HSP70 mRNA的表达,表现为急性冷应激初期和慢性冷应激时,肠组织中HSP70 mRNA表达量增加,而急性冷应激后期下降。

In this study,the effects of cold stress on the expression levels of HSP70 in duodenum,jejunum,ileum and cecum were studied.120 1-day-old male chickens were kept under the temperature of （30± 2） ℃ and were given commercial diet and water.When the chickens were 15 days old,they were randomly divided into 12 groups on average and were kept under the temperature of （12±1） ℃ for acute and chronic cold stress.The duration of acute cold stress were 0,1,3,6,12 and 24 h,and the duration of chronic cold stress were 5,10 and 20 d.0 h was the control group for the acute cold stress,and there were three control groups for chronic cold stress.After the cold stress treatment,the tissues were collected for real-time PCR assessment.The results showed that at the beginning of the acute cold stress and chronic cold strss,HSP70 expression levels were increased significantly（P0.01）,but at the end of acute cold stress,the expression levels were decreased significantly（P0.01）.The study suggested that HSP70 expressioin levels could be influenced by the cold stress and the expression levels be different by different tissues or different durations of the cold exposure.