猪源乳酸芽孢杆菌的筛选与鉴定

Selection and identification of lactic acid bacillus from piglet manure

从健康仔猪粪中分离对仔猪黄白痢有一定防治作用的产乳酸芽孢杆菌。采用普通营养培养基37℃恒温培养24h分离,将分离到的菌进行抑菌、耐酸、耐胆盐试验,并用相差显微镜对其进行形态特征观察,以及经生理生化试验鉴定。结果表明,两菌株经抑菌试验表明对埃希氏大肠杆菌、伤寒沙门氏菌等均有较强的抑制作用,并且能够抵抗高浓度胃酸、胆盐的不良环境,具有较高的存活数。推断:Y4为凝结芽孢杆菌(NRS14)和Y6为凝结芽孢杆菌(NRS172)。

Experiments were conducted to select lactic acid bacillus from piglet manure effect prevention and treatment of yellow and white scour of piglets by common nutrition medium.Using common nutrition medium for 24 h at a constant temperature of 37 ℃.And then the strains were capable of inhibiting the growth of Salmonella typhi and Escheichia coli through antibacterial experiment.And then the strains＇ morphological characteristics were observed by the phase contrast microscope,through its physiology and biochemistry experiment.The results showed that two strains were capable of inhibiting the growth of Salmonella typhi and Escheichia coli through antibacterial experiment,and they had high numbers of survival at resist adverse environment of high concentration gastric acid and biliary salt.Conclusion：Y4 was identified to be Bacillus coagulants （NRS 14） and Y6 was identified to be Bacillus coagulants （NRS 172）.