黄芪对缺血再灌注心肌保护作用机制的实验研究

An experimental study on cardioprotective effect of astragalus membranaceus on ischemic/reperfusion my-ocardium in isolated rabbit hearts

目的 :探讨黄芪的心肌保护作用机制。方法 :应用兔离体心肌缺血再灌注损伤模型 ,在Thomas液中加入黄芪注射液 ,检测冠脉流量、冠脉回流液中肌钙蛋白 T和腺苷释放、心肌组织中的MDA及外部 5′ 核苷酸酶的活性。结果 :黄芪能有效地保护外部 5′ 核苷酸酶的活性 (P <0 .0 5 ) ;能使腺苷释放明显增加 (P <0 .0 5 ) ;能使冠脉流量明显增加 (P <0 .0 5 ) ,能明显降低冠状循环阻力 (P <0 .0 5 ) ;能使MDA含量明显减少 (P <0 .0 5 ) ;能使肌钙蛋白 T释放明显减少 (P <0 .0 5 )。结论 :在心肌缺血再灌注过程中 ,保护心肌细胞外部 5′ 核苷酸酶的活性 ,使内源性腺苷释放明显增加可能是黄芪减轻心肌缺血再灌注损伤的重要机制之一。

OBJECTIVE :TostudythecardioprotectiveeffectandthemechanismofAstragslusmembranaceusduringischemiaand reperfusion .METHODS :Theexperimentalmyocardialischemia/reperfusionmodelofisolatedrabbitwasused .Astragaliinjectionwas giveninThomas hospitalcardioplegicsolution .Thecoronaryflow ,troponin Tandadenosineofeffuent,ecto 5′ nucleotidaseactivity andMDAinmyocardialtissueweremeasured .RESULTS :Astragaliinjectioneffectivelyprotectedecto 5′ nucleotidaseactivity (P < 0 .0 5 ) ,significantlyaugmentedadenosinerelease (P <0 .0 5 ) ,greatlyenhancedcoronaryflow (P <0 .0 5 ) ,largelyreducedtheamount ofMDA (P <0 .0 5 ) ,significantlydecreasedtheamountoftroponin T (P <0 .0 5 ) .CONCLUSION :ThemechanismofAstragalus membranaceusincardioprotectionmaybetheaugmentationofendogenousadenosinereleasethroughprotectingecto 5 nucleotidase activity .Duringthecourseofischemiaandreperfusion ,Astragalusmembranaceusaugmentedendogenousadenosinereleasethrough protectingecto 5′ nucleotidaseactivity .Itmaybeoneoftheimportantmechanismsofattenuatingmyocardialischemia/reperfusionin jury .