糖类对羊血超氧化物歧化酶活性的影响

Effect of Carbohydrates on Activities of Superoxide Dismutase Extracted from Sheep Blood

目的:研究糖类对羊血超氧化物歧化酶(SOD)活性影响关系。方法:以离子交换层析法制备的羊血SOD为原料,加入不同浓度的麦芽糖、蔗糖、乳糖、海藻糖、D-葡萄糖、D-果糖、D-木糖和D-甘露糖,25℃分别保温4、24、48、72h和96h后,利用邻苯三酚自氧化法测定SOD酶活力,并计算相对酶活力。结果:海藻糖对羊血SOD有明显的激活和保护作用,高浓度的麦芽糖和低浓度的乳糖有一定稳定作用,其余糖类对SOD活性影响不大。结论:不同糖类在不同浓度下对羊血SOD活性影响不同。

The activity of sheep blood superoxide dismutase（SOD） purified by DEAE Sephadex A-50 column chromatography was determined by pyrogallo autoxidation method after 4,24,48,72 h and 96 h of 25 ℃ incubation in the respective presence of maltose,sucrose,lactose,fucose,glucose,D-fructose,D-trehalose,D-xylose and D-mannose under varying concentration conditions,and the corresponding relative enzyme activities were calculated.Trehalose had a remarkable activating and protecting effect on sheep SOD.High concentrations of maltose and low concentrations of lactose were conducive to stabilizing the enzyme,and other sugars tested had unobvious effect on the enzyme.Therefore,different kinds and amounts of sugar have different effects on sheep SOD.