花生根细胞悬浮培养制备白藜芦醇条件的优化

Optimization of Suspension Culture Conditions for Preparing Resveratrol in Peanut Root Cells

以花生根愈伤组织为材料,采用二次通用旋转组合试验设计,优化花生根细胞悬浮培养产白藜芦醇的条件,探讨影响花生根细胞悬浮培养产白藜芦醇的最佳工艺参数。当摇床转速为130r/min、接种量为38.00g/L、pH5.80、蔗糖质量浓度为40.00g/L时,培养15d,花生根细胞增殖倍数最大为(4.71±0.04)倍,白藜芦醇产量达到907.3μg/g。

Quadratic rotation combinatorial design was utilized to optimize suspension culture conditions for resveratrol production by peanut root callus tissue.Under the following optimized conditions：shaker rational speed 130 r/min;inoculum size 38.00 g/L;pH 5.80;and sucrose concentration 40.00 g/L,the maximum proliferation fold of suspension cells was 4.71±0.04,and the resveratrol production reached 907.3 μg/g after 15-day incubation.