甜荞苯丙氨酸解氨酶基因PAL的克隆及序列分析

Cloning and Sequence Analysis of Phenylalanine Ammonia-lyase(PAL) Gene from Fagopyrum esculentum

利用RT-PCR技术,首次从甜荞(Fagopyrum esculentum)中克隆得到苯丙氨酸解氨酶基因(PAL)的cDNA ORF序列,命名为FePAL。该序列长2169bp,编码722个氨基酸,与其他植物PAL基因同源性较高,为80%～97%,其推导的氨基酸序列含有PAL酶活性中心特征序列GTITASGDLVPLSYIA和多个脱氨基、催化活性位点。系统发育树表明,甜荞PAL基因与苦荞PAL基因聚类关系最近。

The cDNA ORF sequence of phenylalanine ammonia-lyase gene（FePAL） from Fagopyrum esculentum was cloned by RT-PCR.The cDNA sequence was 2169 bp in length,encoding 722 amino acids and had a high homology（80%-97%） with PAL genes from other plants.The deduced amino acid sequence of FePAL contained the typical sequence（GTTTASGDLVPLSYIA） in PAL activity center region,deamination residues and catalytic active sites.Phylogenetic tree analysis revealed that FePAL was most closely related to the PAL of Fagopyrum tataricum.