摘要
利用RT-PCR技术,首次从甜荞(Fagopyrum esculentum)中克隆得到苯丙氨酸解氨酶基因(PAL)的cDNA ORF序列,命名为FePAL。该序列长2169bp,编码722个氨基酸,与其他植物PAL基因同源性较高,为80%~97%,其推导的氨基酸序列含有PAL酶活性中心特征序列GTITASGDLVPLSYIA和多个脱氨基、催化活性位点。系统发育树表明,甜荞PAL基因与苦荞PAL基因聚类关系最近。
The cDNA ORF sequence of phenylalanine ammonia-lyase gene(FePAL) from Fagopyrum esculentum was cloned by RT-PCR.The cDNA sequence was 2169 bp in length,encoding 722 amino acids and had a high homology(80%-97%) with PAL genes from other plants.The deduced amino acid sequence of FePAL contained the typical sequence(GTTTASGDLVPLSYIA) in PAL activity center region,deamination residues and catalytic active sites.Phylogenetic tree analysis revealed that FePAL was most closely related to the PAL of Fagopyrum tataricum.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第7期255-257,共3页
Food Science
基金
四川省科技厅科技攻关项目(04NG001-015
2006Z08-012)
关键词
甜荞
苯丙氨酸解氨酶基因
克隆
序列分析
Fagopyrum esculentum
phenylalanine ammonia-lyase gene
cloning
sequence analysis