北冬虫夏草DNA提取方法的比较

Comparison of genomic DNA extraction from Cordyceps militaris

运用CTAB法、CTAB改良法1、CTAB改良法2、氯化苄法、裂解法、改良SDS法、改良尿素法等7种方法提取北冬虫夏草基因组DNA,发现CTAB改良法2(提纯时加入低浓度乙醇)提取的DNA的OD260/OD280都在1.9以上,能获得清晰的PCR扩增图谱,能被HindⅢ酶切消化。

This study is an attempt to compare the DNA extraction methods of 7 kinds of extraction： CTAB method,improved CTAB method 1,improved CTAB method 2,benzyl chloride method,lysis method,improved SDS method,and improved urea method so as to isolate high quality genomic DNA from Cordyceps militaris enriching polysaccharides and protein.The results showed that the extraction effect of the third method（improved CTAB method 2）（added low concentration ethanol at purification） was the best.The value of OD260/OD280 was over 1.90,the DNA was amplifiable in the polymerase chain reaction（PCR） and completely digestible with restriction endonucleases HindⅢ.