猪DECR1基因SNPs筛选及其多态性分析

SNPs of DECR1 gene and its genetic polymorphism in pigs

以大白猪、长白猪和杜洛克3个猪种共327头为试验材料,对2,4-dienoyl-CoA reductase 1(DECR1)基因第6至第10外显子区域进行SNPs筛选,分析SNP突变位点的遗传多态性;采用比较基因组方法,根据人DECR1基因全长序列设计引物,筛选猪DECR1基因的SNP,经PCR-SSCP技术和测序检测,发现Exon6和Exon8区域各存在1个SNP突变位点(C/T,C/G),但Exon6区域的SNP突变是无义突变,而Exon8的是错义突变;在对Exon8 SNP的遗传结构分析中,3个猪种中均检测到CC、CG、GG 3种基因型,说明猪群在该位点均具有丰富的遗传多态性。

Three hundred and twenty seven Yorkshire,Landrace and Duroc pigs were analysed to screen the SNPs in 6-10 Exon of mitochondrial 2,4-dienoyl-CoA reductase 1（DECR1） gene and analyse its genetic structure.The resluts showed： the primers were designed accroding to human DECR1 gene by means of genome comparison,2 SNPs were screened out in Exon6 and Exon8 by PCR-SSCP method,but mutation in Exon6 loic belonged to nonsense mutation,mutation in Exon8 loci belong to missense mutation.The polymorphism of the SNP in Exon8 were studied and 3 genotypes（CC,CG,GG） were tested in 3 breeds.There were abundant genetic polymorphism in Exon8 loci.